Bio 6B Lab Resume

If want apply for a lab position in the future, you might be asked to list your lab skills and experience. The list below shows the lab techniques anc concepts you should know by the time you finish this course. Ideally, you should be able to explain everything on this list, in terms of how you’d do the lab work, why you’d do it that way, and what the results would mean (including all the controls). Feel free to copy the list and use it as part of your resume, but please be honest: do you really understand these things? With that in mind, it's a good idea to review this list even if you're not applying for a lab position. It's another way to assess your own progress in learning biology.

General Concepts & Techniques

Protein techniques:

  • SDS-PAGE
  • Fluorimetric protein assay
  • Hydrophobic interation chromatography

DNA techniques:

  • Restriction digests
  • Plasmid DNA purification
  • Fluorimetric DNA assays
  • DNA electrophoresis
  • Polymerase chain reaction (including primer design and using PCR to identify polymorphisms)

Working with E. coli:

  • Sterile technique; plate & liquid cultures
  • Selective media & antibiotics
  • Antibiotic resistance
  • Plating techniques for colonies
  • Plating techniques for phage
  • Conjugation
  • Transformation

Plasmid concepts:

  • Antibiotic resistance genes
  • AraBAD/GFP operon and controlling gene expression with differential media
  • Origin of replication and origin of transfer
  • Circular vs. linear DNA on gels
  • Restriction maps compared to gel results

Bioinformatics:

  • Identifying restriction sites and making restriction maps
  • Identifying open reading frames and predicting amino acid sequences
  • Using BLAST to search a database for similar sequences and for producing alignments
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